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CN 34-1304/RISSN 1674-3679

Volume 28 Issue 11
Nov.  2024
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WANG Xueqi, HE Na. Systematic review of CRISPR applied to point-of-care nucleic acid detection[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2024, 28(11): 1344-1348. doi: 10.16462/j.cnki.zhjbkz.2024.11.016
Citation: WANG Xueqi, HE Na. Systematic review of CRISPR applied to point-of-care nucleic acid detection[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2024, 28(11): 1344-1348. doi: 10.16462/j.cnki.zhjbkz.2024.11.016

Systematic review of CRISPR applied to point-of-care nucleic acid detection

doi: 10.16462/j.cnki.zhjbkz.2024.11.016
Funds:

National Key Research and Development Program of China 2022YFC2304902

Key Disciplinary Program of Shanghai Public Health Action Plan GWVI-11.1-05

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  • Corresponding author: HE Na, E-mail: nhe@fudan.edu.cn
  • Received Date: 2024-05-08
  • Rev Recd Date: 2024-08-19
  • Available Online: 2024-12-23
  • Publish Date: 2024-11-10
  •   Objective  To understand the current research status of clustered regularly interspaced short palindromic repeats (CRISPR) applied to nucleic acid detection.  Methods  In this study, we systematically reviewed related literatures published on databases of PubMed, BioRxiv, and MedRxiv using the keywords "CRISPR", "nucleic acid detection", "point-of-care testing" or "POCT".  Results  According to the inclusion and exclusion criteria, a total of 17 publications were selected. We summarized and analyzed the information of CRISPR-associated protein, amplification method, sensitivity, specificity, detection time and other aspects. Results suggested that when combined with nucleic acid amplification methods, such as loop-mediated isothermal amplification (LAMP) or recombinase polymerase amplification (RPA), the sensitivity of CRISPR was comparable to real time quantitative polymerase chain reaction (RT-qPCR).  Conclusions  The process of CRISPR can be completed within 30 to 100 minutes, with results that can be read by the naked eye. Additionally, the cost of detection is comparable to the antigen test, making it particularly suitable for large-scale point-of-care (POC) nucleic acid detection in resource limited areas.
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