血浆中微量元素水平与HCV感染及肝功能情况关系的研究

谢超楠; 吴梦平; 黄鹏; 岳明; 彭志行; 喻荣彬; 王洁; 张云

doi:10.16462/j.cnki.zhjbkz.2016.09.003

血浆中微量元素水平与HCV感染及肝功能情况关系的研究

doi: 10.16462/j.cnki.zhjbkz.2016.09.003

谢超楠¹,
吴梦平¹,
黄鹏¹,
岳明²,
彭志行¹,
喻荣彬¹,
王洁^3, ,,
张云^1,4, ,

1. 南京医科大学公共卫生学院流行病学系, 江苏南京 210029;
2. 南京医科大学第一附属医院感染科, 江苏南京 210029;
3. 南京医科大学护理学院护理学基础系, 江苏南京 210029;
4. 南京军区军事医学研究所流行病与微生物研究所, 江苏南京 210002

基金项目:

国家自然科学基金（81473028，81502853）；江苏高校“青蓝工程”（2016年）；江苏省自然科学基金（BK20151026）；江苏省重点学科建设项目“护理学”（JX10617801）

详细信息

作者简介:
谢超楠(1991-),女,安徽淮北人,在读硕士研究生。主要研究方向:传染病流行病学。

中图分类号: R512.63;R181.1
计量
- 文章访问数: 217
- HTML全文浏览量: 24
- PDF下载量: 33
- 被引次数: 0
出版历程
- 收稿日期: 2016-03-14
- 修回日期: 2016-07-24

Study on association of plasma trace elements concentration with HCV infection and liver function

1. Department of Epidemiology, School of Public Health, Nanjing Medical University, Nanjing 210029, China;
2. Department of Infectious Diseases, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China;
3. Department of Fundamental Nursing, School of Nursing, Nanjing Medical University, Nanjing 210029, China;
4. Institute of Epidemiology and Microbiology, the Institute of Military Medicine of Nanjing Command, Nanjing 210002, China

摘要

摘要: 目的分析丙型肝炎患者血浆中微量元素（铜、铁和锌）含量及其与丙型肝炎病毒（hepatitis C virus, HCV）感染及相关肝功能指标的关系。方法运用电感耦合等离子体质谱法（inductively coupled plasma mass spectrometry, ICP-MS）检测HCV持续感染者（90例）、自限清除者（92例）和对照（99例）血浆中的铜、铁和锌含量，偏相关和多因素Logistic回归分析HCV持续感染者血浆中微量元素水平与HCV病毒载量的关系。结果 HCV持续感染组血浆中锌含量低于对照组，铁含量高于对照组，差异均有统计学意义（均有P<0.05）；以年龄、性别、体质指数（body mass index, BMI）为协变量的偏相关分析结果发现，铁与HCV RNA水平呈负相关（r=-0.263，P=0.016）。以年龄、性别、BMI、各微量元素含量为自变量进行多因素Logistic回归分析，结果显示铁含量升高是HCV持续感染者血浆丙氨酸氨基转移酶（alanine aminotransferase，ALT）水平升高的危险因素（OR=1.001，95% CI:1.000～1.002，P=0.030）。结论微量元素铜、铁、锌可能和HCV感染转归相关，其中铁水平升高是HCV感染慢性化的独立危险因素。
- 肝炎,丙型, 慢性 /
- 铁 /
- 病毒载量
Abstract: Objective To explore the association of plasma microelements (Cu, Fe and Zn) concentration with hepatitis C virus (HCV) infection and liver function in hepatitis C patients. Methods In a case-control study, 281 participants, including 90 patients with chronic hepatitis C, 92 HCV natural clearance subjects and 99 healthy controls, were investigated. The subjects' plasma trace elements concentration was detected by inductively coupled plasma mass spectrometry (ICP-MS). And partial correlation analysis and multivariable logistic regression analysis were used to explore the relationship of microelements concentration and HCV viral load in patients with chronic HCV infection. Results Compared with healthy controls, the level of zinc was decreased, however, the level of iron was increased in patients with chronic HCV infection (all P<0.05). After controlling for age, sex and BMI, the HCV viral load was negatively correlated with plasma iron concentration (r=-0.263, P=0.016). The Logistic regression showed that iron was independently associated with ALT values in patients with persistent HCV infection (OR=1.001,95% CI:1.000-1.002,P=0.030). Conclusions Trace elements (Cu, Fe and Zn) may be associated with HCV infection outcomes. Especially, iron level is an independent risk factors of chronic HCV infection.
- Hepatitis C, chronic /
- Iron /
- Viral load

HTML全文

参考文献(17)

Felmlee DJ, Hafirassou ML, Lefevre M, et al. Hepatitis C virus, cholesterol and lipoproteins-impact for the viral life cycle and pathogenesis of liver disease [J]. Viruses, 2013,5(5):1292-1324.

Lavanchy D. The global burden of hepatitis C [J]. Liver Int, 2009,29(Suppl 1):74-81.

刘媛,赵杰,龙璐,等. HCV慢性感染及自发清除对血清微量元素含量的影响及其与血清白蛋白和HCV病毒载量的关联研究 [J]. 中华微生物学和免疫学杂志, 2013,33(12):922-926.

Fillebeen C, Pantopoulos K. Iron inhibits replication of infectious hepatitis C virus in permissive Huh7.5.1 cells [J]. J Hepatol, 2010,53(6):995-999.

王敏,乐晓华,韩红星,等. HCV-RNA 病毒载量和肝脏病理的关系 [J]. 实用医学杂志, 2006,22(13):1513-1514.

陈继梅,许叶虹,丁雪芳. 慢性丙型肝炎感染者多项指标动态监测结果分析 [J]. 中华疾病控制杂志, 2013,17(6):548-550.

董爱莲,胡玉琳. 铁代谢紊乱和其他致病因素介导的肝氧化损伤及抗氧化保护策略研究进展 [J]. 生物物理学报, 2012,28(4):307-316.

Sikorska K, Stalke P, Izycka-Swieszewska E, et al. The role of iron overload and HFE gene mutations in the era of pegylated interferon and ribavirin treatment of chronic hepatitis C [J]. Med Sci Monit, 2010,16(3):CR137-143.

Mueller S. Increased iron in HCV infection: collateral damage or antiviral defense? [J]. J Hepatol, 2010, 53(6):990-992.

Fillebeen C, Pantopoulos K. Hepatitis C virus infection causes iron deficiency in Huh7.5.1 Cells [J]. PLoS One, 2013,8(12):e83307.

Arain SA, Kazi TG, Afridi HI, et al. Estimation of copper and iron burden in biological samples of various stages of Hepatitis C and liver cirrhosis patients [J]. Biol Trace Elem Res, 2014,160(2):197-205.

杨金霞,刘海军,孙晋民,等. 甲型乙型丙型肝炎患者血清微量元素含量测定 [J]. 山西医药杂志, 2010,39(10):1011-1012.

刘媛,赵杰,沈弢,等. 慢性HCV/HIV合并感染对血清锌的影响 [J]. 营养学报, 2014,36(1):40-44.

Guo CH, Chen PC, Lin KP, et al. Trace metal imbalance associated with oxidative stress and inflammatory status in anti-hepatitis C virus antibody positive subjects [J]. Environ Toxicol Pharmacol, 2012,33(2):288-296.

孙丽萍,王丽英,张晓静,等. 微量元素与丙型病毒性肝炎的研究(3) [J]. 中国公共卫生, 2002,18(10):1223-1224.

Yano M, Ikeda M, Abe K, et al. Comprehensive analysis of the effects of ordinary nutrients on Hepatitis C virus RNA replication in cell Culture [J]. Antimicrob Agents Chemother, 2007,51(6):2016-2027.

Fillebeen C, Rivas-Estilla AM, Bisaillon M, et al. Iron inactivates the RNA polymerase NS5B and suppresses subgenomic replication of Hepatitis C virus [J]. J Bio Chem, 2005,280(10):9049-9057.

施引文献

资源附件(0)

访问统计