Abstract: Objective To analyze the epidemiology characteristic of influenza by cases from 13 sentinel sites for hospital-based influenza surveillance in Jiangsu province during 2010-2014 and to explore influenza prevention and control. Methods According to the requirements by “National Influenza (swine flu) Monitoring Plan”, influenza-like cases (ILI) from the sentinel hospitals were collected. The pharyngeal swab specimen were collected for influenza virus RNA detection and virus isolation. Clinical cases were entered into the national influenza network monitoring system. Excel 2007 and PASW(Predictive Analytics Software) 18.0 were used for data analysis. Results Total 123 330 ILI cases were reported in five years. 14 238 influenza virus strain were isolated with a positive rate of 11.54%. Kindergarten and scattered children was the dominant group in all occupations. 0- age group contributed the largest population by age distribution. Conclusions There are two epidemic peaks of influenza each year in Jiangsu province, from January to March and July to October, respectively. In the years of 2010 to 2012, the epidemic flu strains were seasonal A/H3N2 and type B. The main epidemic flu strain switched to seasonal A/H3N2 and A/H1N1 (09pdm) between 2013 and 2014.