Abstract: Objective To investigate the sleep quality of college students from eight universities of Chinese medicine and analyze the possible influencing factors. Methods A stratified cluster sampling method was used to obtain 7 142 college students from 8 universities of traditional Chinese medicine in four regions of China. The Pittsburgh sleep quality index (PSQI) and a self-designed questionnaire were used to carry out a cross-sectional survey. Results Totally 6 534 valid questionnaires were retrieved, boys 1 778 (27.21%), girls 4 756 (72.79%); the mean age was (20.32±1.23) years. The mean score of PSQI was (4.58±2.30), detection rate of insomnia (PSQI ≥ 8) was 10.33%. The score of PSQI of students from cities (4.36±2.32) was better than those from villages and towns (4.64±2.29, t=-3.909, P<0.001). The score of PSQI of students who belonged to extroversion (4.50±2.38) and intermedius type (4.54±2.25) was better than those who belonged to introversion (4.78±2.35, F=6.481, P=0.002). The score of PSQI of students with normal weight(4.52±2.22) was better than those who were underweight (4.64±2.38), overweight (4.89±2.60) or obesity (5.03±2.60, F=5.892, P=0.001). There was no significant difference in PSQI score between different genders(t=-0.005, P=0.996). Multiple regression analysis showed that there were twelve factors which may influence college students' sleep quality like index of pressure, life satisfaction and regular dietary. Conclusions The sleep quality of students from universities of Chinese medicine which was influenced by many factors still needs to be further improved.