Abstract: Objective To analyze the viral pathogenic spectrum and epidemiological characteristics of febrile respiratory syndrome(FRS) in Wuhan City, so as to provide theoretical basis for prevention, control and clinical diagnosis and therapy of FRS. Methods The swabs specimens from 576 patients of FRS were collected from Mar 2012 to Feb 2015, and detected by using real-time fluorescent quantitative polymerase chain reaction (PCR). Results A total of 576 specimens were tested, 248 specimens were positive for viral ncleic acid, and the positive rate was 43.1%. No statistically significant difference was found in the positive detection rate between males and females (χ²=0.371,P=0.542). Among the 248 positive specimens, 208 indicated for single infection and 40 indicated for mix infections. Among the detected respiratory viruses, adenovirus(AdV), rhinovirus(HRV) and influenza A virus(FluA) accounted for the large proportion profile, the rates were 19.4%, 17.3% and 14.1%, respectively. In different age groups, statistically significant differences were found in the infection rates of respiratory syncytial virus B, AdV, Bocavirus(BoV) and co-infection (all P<0.05), the age group of 2-was significantly different from others in the AdV infection and co-infection and the BoV infection was found only in the age group of <2. The different respriratory viruses were prevalent in different epidemic seasons. The detection rates of virus were significantly different in different seasons(χ²=17.427,P=0.001), the detection rate of virus was the lowest in the Spring. Conclusions AdV, HRV and FluA were important pathogens of FRS in Wuhan City, children under age of 5 were high risk population. The composition of pathogenic spectrum was different in different seasons, it was necessary to conduct further surveillance and more in-depth investigation targeted on prevention and control of the febrile respiratory illness caused by these respiratory viruses.