苏州市H5N6亚型高致病禽流感暴发疫情病毒基因特征分析

董泽丰; 雅雪蓉; 王笛; 沈强; 夏瑜

doi:10.16462/j.cnki.zhjbkz.2019.11.023

苏州市H5N6亚型高致病禽流感暴发疫情病毒基因特征分析

doi: 10.16462/j.cnki.zhjbkz.2019.11.023

215004 苏州, 苏州市疾病预防控制中心检验科, 病毒免疫实验室

基金项目:

苏州市“科教兴卫”青年科技项目 KJXW2017051

苏州科技局—民生科技项目 SYS201780

苏州市医学重点学科 SZXK201516

详细信息

通讯作者:
夏瑜, E-mail: xiayusuzhou@hotmail.com

中图分类号: R183.3
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出版历程
- 收稿日期: 2019-03-15
- 修回日期: 2019-08-03
- 刊出日期: 2019-11-10

Genetic characteristics of highly pathogenic avian influenza A(H5N6) virus in Suzhou City

Microbial Laboratory, Suzhou Center for Disease Control and Prevention, Suhou 215004, China

Funds:

Promoting the Health Development by Relying on Science and Education KJXW2017051

Science and Technology Project for Improving People's Livelihood SYS201780

Suzhou Key Medical Discipline SZXK201516

More Information

Corresponding author: XIA Yu, E-mail: xiayusuzhou@hotmail.com

摘要

摘要: 目的对苏州市H5N6亚型高致病禽流感暴发疫情病毒进行全基因测序，分析病毒基因变异情况。方法实时荧光-逆转录聚合酶链反应（real-time fluorescent quantitative reverse transcription-polymerase chain reaction，RT-PCR）方法检测病死禽咽/肛拭子、疑似病例咽拭子标本甲/乙型及各亚型流感病毒核酸。Sanger法对H5N6病毒进行全基因测序并构建HA及NA系统发育树。结果苏州市2018-2019流行季了报告1例人感染H5N6禽流感实验室确诊病例，春节期间W区某地持续出现病死禽经实验室检测确诊为H5N6禽流感疫情。序列分析表明苏州株血凝素（hemagglutinin，HA）及神经氨酸酶（neuramidinase，NA）基因同源性分别为98.01%~100.0%和98.16%~100.0%。A/chicken/Suzhou/WZ01/2019（H5N6）和A/chicken/Suzhou/WZ03/2019（H5N6）均发生了D198N及Q226H耐药突变。苏州H5N6病毒株属2.3.4.4 H5进化分支，HA蛋白切割位点氨基酸序列为RERRRKR↓GLF，为高致病禽流感病毒。结论 H5N6高致病禽流感病毒为新型多源重配病毒且仍在不断进化，苏州株多个基因位点发生漂移；应密切监测耐药突变及耐药毒株的流行并及时采取科学有效处置措施防止对公众健康和养殖业造成重大损害。
- 高致病禽流感 /
- H5N6病毒 /
- 血凝素 /
- 耐药突变 /
- 糖基化
Abstract: Objective To analyze host adaptability and drug resistance variation of highly pathogenic avian influenza (HPAI) A(H5N6) viruses obtained from outbreaks in Suzhou City. Methods Real-time fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR) assay was used for influenza virus detection in pharynx or anus swabs of dead birds and suspected cases. Full genome of H5N6 positive samples were sequenced by using Sanger method. Hemagglutinin (HA) and neuramidinase (NA) phylogenetic trees were constructed by MEGA software. Results A child was laboratory confirmed to have A(H5N6) viruses infection in November 2018. A outbreak of avian flu in poultry in W district during the Spring Festival was laboratory confirmed as a A(H5N6) epidemic situation. Avian-origin H5N6 viruses possessed D198N and Q226H resistance mutations. The homology of HA and NA genes of Suzhou strains were 98.01%-100.0% and 98.16%-100.0%, respectively. These H5N6 strains belonged to 2.3.4.4 H5 clad. Multiple basic amino acid at the cleavage site of HA implied the highly pathogenic characteristics of Suzhou H5N6 strains. Conclusion H5N6 is a kind of malti-sourse reassortment virus, which is still evolving. Multiple loci of Suzhou H5N6 strains of this epidemic were identified to have drifted. The prevalence of drug-resistant mutations should be closely monitored in order to timely take effective measures to prevent serious damage to public health and poultry production industries.
- Avian Influenza /
- H5N6 virus /
- Hemagglutinin /
- Drug-resistance /
- Glycosylation

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图 1 苏州市H5N6禽流感病毒HA和NA基因Neighbour-Joining系统进化树

Figure 1. Phylogenetic tree of HA and NA gene of A(H5N6) viruses in Suzhou City

下载: 全尺寸图片幻灯片

表 1 苏州禽源H5N6禽流感病毒各基因片段核酸一致性最高的序列

Table 1. The best reference sequences hit for avian origin A(H5N6) viruses in Suzhou City

基因片段	最佳参考序列	核酸一致性(%)
PB2	A/Pavo cristatus/Jiangxi/JA1/2016(H5N6)	98.55
PB1	A/Guangxi/13486/2017(H5N6)	99.08
PA	A/Guangxi/31906/2018(H5N6)	98.78
HA	A/Guangxi/13486/2017(H5N6)	99.03
NP	A/Guangxi/13486/2017(H5N6)	99.42
NA	A/Pavo cristatus/Jiangxi/JA1/2016(H5N6)	98.74
MP	A/Guangdong/18SF020/2018(H5N6)	99.22
NS	A/Guangxi/13486/2017(H5N6)	99.20

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