男男同性恋人群治疗前HIV-1流行毒株及其变异研究

路新利; 张玉琪; 邱延超; 李保军; 李岩; 王莹莹; 刘萌; 安宁

doi:10.16462/j.cnki.zhjbkz.2020.12.019

男男同性恋人群治疗前HIV-1流行毒株及其变异研究

doi: 10.16462/j.cnki.zhjbkz.2020.12.019

1.
050021 石家庄，河北省疾病预防控制中心艾滋病所，河北省传染病病原学鉴定分析与流行病学重点实验室
2.
050011 石家庄，石家庄市疾病预防控制中心艾滋病所

基金项目:

河北省自然科学基金 H2016303006

详细信息

通讯作者:
路新利, E-mail:lxlii2009@163.com

中图分类号: R373
计量
- 文章访问数: 653
- HTML全文浏览量: 394
- PDF下载量: 41
- 被引次数: 0
出版历程
- 收稿日期: 2020-09-30
- 修回日期: 2020-11-04
- 刊出日期: 2020-12-10

HIV-1 epidemic strains and genetic variation among therapy-naive men who have sex with men

1.
Department of AIDS Research, Hebei Key Laboratory of Pathogen and Epidemiology of Infectious Disease, Hebei Provincial Center for Disease Control and Prevention, Shijiazhuang 050021, China
2.
Department of AIDS Research, Shijiazhuang Center for Disease Control and Prevention, Shijiazhuang 050011, China

Funds:

Provincial Natural Science Foundation of Hebei H2016303006

More Information

Corresponding author: LU Xinli, E-mail:lxlii2009@163.com

摘要

摘要: 目的了解掌握男男同性恋者(men who have sex with men, MSM)人群治疗前人类免疫缺陷病毒Ⅰ型(human immunodeficiency virus type one, HIV-1)流行毒株与基因变异特点。方法使用In-house方法进行耐药基因型检测，通过系统进化分析和HIV-1耐药数据库比对确定基因型和基因变异情况。结果石家庄市MSM人群中共有5种HIV-1基因型毒株在流行，CRF01_AE是最主要流行毒株，占到56.8%(25/44)，其次是CRF07_BC 29.5%(13/44)，URFs 9.1%(4/44)，B亚型2.3%(1/44)和CRF55_01B 2.3%(1/44)。URFs主要包括CRF01_AE/B和CRF01_AE/C两种重组模式。χ²检验显示不同基因型毒株在婚姻状况、年龄和首次CD4⁺ T淋巴细胞中的分布差异均有统计学意义(均有P < 0.05)。发现1例MSM在HIV-1 NNRT编码区225位点处发生了耐药基因突变(P225H)，原发耐药率为2.3%(1/44)，可导致依非韦伦(EFV)和奈韦拉平(NVP)中度耐药。结论加强MSM人群中HIV-1流行和耐药监测研究对控制本市HIV疫情发展具有重要意义。
- 人类免疫缺陷病毒Ⅰ型 /
- 基因型 /
- 男男同性恋者 /
- 变异
Abstract: Objective To explore HIV-1 genotypes and genetic variation among therapy-naive men who have sex with men (MSM) in Shijiazhuang. Methods HIV-1 genotypic resistance assay was carried out using In-house method. HIV-1 genotypes and drug resistance were analyzed by phylogenetic analysis and submitting the sequences to the Stanford University Network HIV-1 database. Results Five HIV-1 subtypes were found among MSM in Shijiazhuang. Of them, CRF01_AE was the most frequent, accounting for 56.8% (25/44), followed by CRF07_BC 29.5% (13/44), URFs 9.1% (4/44), B 2.3% (1/44) and CRF55_01B 2.3% (1/44). URFs mainly included CRF01_AE/B and CRF01_AE/C. χ² -test verified that HIV-1 genotype distribution showed significant difference (P < 0.05) in marital status, age and first CD4 groups, respectively. The rate of HIV-1 primary drug resistance was 2.3% (1/44). P225H mutation was found in NNRT gene coding region, and one MSM with P225H occurred intermediate resistance to EFV and NVP. Conclusion It is critical for us to take measures to monitor HIV-1 epidemic and drug resistance.
- Human immunodeficiency virus type one /
- Genotype /
- Men who have sex with men /
- Mutation

HTML全文

图 1 2018年石家庄市基于HIV-1 pol基因序列的Neighbor-joining系统进化树

Figure 1. Neighbor-joining tree basing on HIV-1 pol sequences in Shijiazhuang City in 2018

下载: 全尺寸图片幻灯片

图 2 URFs jpHMM-HIV基因重组图谱

Figure 2. Recombinant maps of URFs using jpHMM-HIV

下载: 全尺寸图片幻灯片

表 1 石家庄市MSM抗病毒治疗前HIV-1基因亚型分布

Table 1. HIV-1 genotype distribution among naive MSM in Shijiazhuang

特征	例数(%)	基因型				χ²值	P值
特征	例数(%)	01_AE	07_BC	URFs	Other	χ²值	P值
婚姻状态						33.000	0.001
未婚	30(68.2)	19	7	3	1
已婚	13(29.5)	5	6	1	1
离婚	1(2.3)	1	0	0	0
教育水平						12.000	0.101
高中以下	20(45.5)	9	8	3	0
大学以上	24(54.5)	16	5	1	2
年龄(岁)						33.600	0.001
19~	15(34.1)	8	5	2	0
26~	28(63.6)	17	7	2	2
>49	1(2.3)	0	1	0	0
首次CD4⁺T淋巴细胞(个/ mm³)						23.700	0.008
＜200	10(22.7)	4	2	2	2
200~	27(61.4)	4	8	2	0
>500	7(15.9)	17	3	0	0
合计	44(100.0)	25	13	4	2
注：其他基因型包括1例CRF55_01B和1例B。

下载: 导出CSV

参考文献(10)

[1]	陈素良, 马琳, 路新利, 等.河北省1989-2013年HIV/AIDS死亡与HAART关系的分析[J].中华流行病学杂志, 2015, 36(5):460-464. DOI: 10.3760/cma.j.issn.0254-6450.2015.05.011. Chen SL, Ma L, Lu XL, et al. Effect of highly active anti-retroviral therapy on reducing HIV/AIDS related death in Hebei Province, 1989-2013[J]. Chin J Epidemiol, 2015, 36(5):460-464. DOI: 10.3760/cma.j.issn.0254-6450.2015.05.011.
[2]	Lu XL, Zhao HR, Zhang YQ, et al. HIV-1 drug-resistant mutations and related risk factors among HIV-1-positiveindividuals experiencing treatment failure in Hebei Province, China[J]. AIDS Res Ther, 2017, 14(1):4. DOI: 10.1186/s12981-017-0133-3.
[3]	路新利, 康现江, 赵宏儒, 等.不同感染途径HIV-1患者治疗后的耐药突变及影响因素分析[J].解放军医学杂志, 2015, 40(7):591-594. DOI: 10.11855/j.issn.0577-7402.2015.07.16 Lu XL, Kang XJ, Zhao HR, et al. Drug resistance mutations and their influencing factors in the patients infected with HIV-1 through different routes after antiretroviral therapy[J]. Med J Chin PLA, 2015, 40(7):591-594. DOI: 10.11855/j.issn.0577-7402.2015.07.16
[4]	王险峰, 李峰, 刘晓松, 等.石家庄市2015年新确诊HIV-1感染者流行毒株的亚型分布及原发性耐药分析[J].中国艾滋病性病, 2018, 173(02):120-124. DOI: 10.13419/j.cnki.aids.2018.02.04. Wang XF, Li F, Liu XS, et al. Genetic characteristics and primary drug resistance of HIV-1 among newly-diagnosed HIV-infected individuals in Shijiazhuang City of Hebei Province in 2015[J]. Chin J AIDS STD, 2018, 173(02):120-124. DOI: 10.13419/j.cnki.aids.2018.02.04.
[5]	刘淑君, 刘丽花, 党静, 等.石家庄市HIV/AIDS疫情分析[J].现代预防医学, 2010, 37(16):3153-3154. http://www.cnki.com.cn/Article/CJFDTotal-XDYF201016062.htm Liu SJ, Liu LH, Dang J, et al. Analysis on the HIV/AIDS epidemic in Shijiazhuang[J]. Modern Prevent Med, 2010, 37(16):3153-3154. http://www.cnki.com.cn/Article/CJFDTotal-XDYF201016062.htm
[6]	Lu XL, Kang XJ, Liu YJ, et al. HIV-1 molecular epidemiology among newly diagnosed HIV-1 individuals in Hebei, a low HIV prevalence province in China[J]. PLoS One, 2017, 12(2):e0171481. DOI: 10.1371/journal.pone.0171481.
[7]	郑敏娜, 周宁, 宁铁林, 等.天津市男男性行为人群HIV感染者毒株亚型和成簇特点[J].中华疾病控制杂志, 2018, 22(12):1252-1256. DOI: 10.3760/cma.j.issn.0254-6450.2016.08.018. Zheng MN, Zhou N, Ning TL, et al. Analysis on the Characteristics of HIV genetic diversity and clusters among MSMs in Tianjin[J]. Chin J Dis Control Prev, 2018, 22(12):1252-1256. DOI: 10.3760/cma.j.issn.0254-6450.2016.08.018.
[8]	孙伟东, 姜天俊, 黄辉煌, 等.北京地区HIV-1感染者治疗前耐药特征及2年抗病毒治疗效果研究[J].国际病毒学杂志, 2020, 27(3):224-227. DOI: 10.3760/cma.j.issn.1673-4092.2020.03.011 Sun WD, Jiang TJ, Huang HH, et al. Characteristics of pretreatment drug resistance in HIV-1 infected patients in Beijing and the efficacy of two-year antiretroviral therapy[J]. International Journal of Virology, 2020, 27(3):224-227. DOI: 10.3760/cma.j.issn.1673-4092.2020.03.011
[9]	Lu XL, Kang XJ, Chen Sl, et al. HIV-1 genetic diversity and transmitted drug resistance among recently infected individuals at men who have sex with men sentinel surveillance points in Hebei Province, China[J]. AIDS Res Hum Retroviruses, 2015, 31(10):1038-1045. DOI: 10.1089/AID.2015.0160.
[10]	Lu XL, Chen SL, Zhao HR, et al. Baseline investigation of HIV-1 primary drug resistance among newly diagnosed treatment-naive HIV-1 individuals in Hebei, China[J]. AIDS Res Hum Retroviruses, 2018, 34(12):1083-1089. DOI: 10.1089/AID.2018.0142.