Infection status of tick-borne encephalitis virus and Alongshan virus in tick-bite patients in Harbin from 2022 to 2023
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摘要:
目的 探究2022―2023年在黑龙江省森工总医院就诊的哈尔滨市蜱叮咬人群中森林脑炎(tick-borne encephalitis virus, TBEV)、阿龙山病毒(Alongshan virus, ALSV)的感染情况。 方法 利用2022―2023年在黑龙江省森工总医院就诊的哈尔滨市蜱叮咬人群的检测数据，通过流行病学调查和系统发育树构建，探究森林脑炎和阿龙山热的感染情况。 结果 2022―2023年在黑龙江省森工总医院就诊的哈尔滨市蜱叮咬的人数为159人，其中阿城区和宾县分别占该院总哈尔滨市蜱叮咬就诊人群的30.19%(48/159)和16.35%(26/159)；哈尔滨市的主城区为松北区、道外区、道里区、南岗区、香坊区，分别有7、1、3、7、7名人员被蜱叮咬后到黑龙江省森工总医院就诊。在该院哈尔滨市蜱叮咬就诊人群中，森林脑炎和阿龙山热病毒的感染率分别为37.74%(60/159)和10.06%(16/159)。在该院就诊的哈尔滨市蜱叮咬的人群中检出的TBEV为远东亚型。 结论 在哈尔滨市的市区和周边县(市)均有被蜱叮咬的风险，并可能感染TBEV和ALSV；除森林脑炎和阿龙山热外，哈尔滨市还可能流行莱姆病、斑点热等其他蜱媒疫病，应加强哈尔滨市蜱传疾病流行情况的监测，广泛宣传预防蜱媒疫病的知识。 Abstract:Objective To explore the infection of tick-borne encephalitis virus (TBEV) and Alongshan virus (ALSV) in tick bites patients in Harbin from 2022 to 2023 in the General Hospital of Forest Industry of Heilongjiang Province. Methods Through epidemiological studies and phylogenetic tree construction, the prevalence of TBEV and ALSV was investigated based on the detection data of tick bitten people in Heilongjiang Red Cross Sengong General Hospital from 2022 to 2023. Results From 2022 to 2023, there were 159 tick bite patients in Harbin who visited Heilongjiang Red Cross Sengong General Hospital. Acheng District and Bin County had more tick-bite people in Harbin, accounting for 30.19% (48/159) and 16.35% (26/159) of all Harbin tick-bite people, respectively. In Songbei District, Daowai District, Daoli District, Nangang District and Xiangfang District, which are the main urban areas of Harbin, 7, 1, 3, 7 and 7 people were bitten by ticks and went to Heilongjiang Red Cross Sengong General Hospital for treatment, respectively. The prevalence of TBEV and ALSV in these patients was 37.74% (60/159) and 10.06% (16/159), respectively. The subtype of TBEV prevalent in Harbin was TBEV-FE. Conclusions There is a risk of tick bites in the Harbin area, both in the city and in the surrounding counties, and can be infected with TBEV and ALSV. In addition to TBE and ALS, other tick-borne diseases, such as Lyme disease and spotted fever, may be prevalent in the Harbin area, and there is a need to strengthen the monitoring of the prevalence of tick-borne diseases in the Harbin and to widely publicize knowledge on the prevention of tick-borne diseases. -
Key words:
- Tick-borne diseases /
- Tick-borne encephalitis virus /
- Alongshan virus /
- Fever
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表 1 森林脑炎病毒和阿龙山病毒PCR扩增使用的引物序列
Table 1. Amplification primer sequences of tick-borne encephalitis virus and alongshan virus
病原体
Pathogen引物名称
Primer name引物序列(5′-3′)
Primer sequence产物长度
Product length/bp森林脑炎病毒
Tick-borne encephalitis virusF1001 GGGAYTTTGTTACTGGCACTC 534 R1535 TCAACRCCGCTGGCTACTC F1096 ATGGATGTGTGGCTTGACTC 364 R1459 GAAACCGTGARGGATGCTGT 阿龙山病毒
Alongshan virus1476F CCGTCCATGAGGCTTTTACCG 421 1929R GCCCAGTCTCTGCTGATAAGGA 1509F TGCCAAAGCACTGGTACGAC 
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[1] Díaz-Sánchez S, Estrada-Peña A, Cabezas-Cruz A, et al. Evolutionary insights into the tick hologenome [J]. Trends Parasitol, 2019, 35(9): 725-737. DOI: 10.1016/j.pt.2019.06.014. [2] Zhao GP, Wang YX, Fan ZW, et al. Mapping ticks and tick-borne pathogens in China [J]. Nat Commun, 2021, 12(1): 1075. DOI: 10.1038/s41467-021-21375-1. [3] 孙世伟, 陆振华, 龙泳, 等. 病毒宏基因组学在蜱媒病毒研究中的应用进展[J]. 中华疾病控制杂志, 2024, 28(3): 357-360. DOI: 10.16462/j.cnki.zhjbkz.2024.03.018.Sun SW, Lu ZH, Long Y, et al. Application progress of virus metagenomics in tick-borne virus research [J]. Chin J Dis Control Prev, 2024, 28(3): 357-360. DOI: 10.16462/j.cnki.zhjbkz.2024.03.018. [4] 张殿鸿, 张志学, 王耀明, 等. 黑龙江省森林脑炎疫情趋势分析[J]. 疾病监测, 2000, 15(2): 57-58. DOI: 10.3784/j.issn.1003-9961.2000.02.012.Zhang DH, Zhang ZX, Wang YM, et al. Analysis on epidemic trend of forest encephalitis in Heilongjiang Province [J]. Dis Surveill, 2000, 15(2): 57-58. DOI: 10.3784/j.issn.1003-9961.2000.02.012. [5] Lindquist L, Vapalahti O. Tick-borne encephalitis [J]. Lancet, 2008, 371(9627): 1861-1871. DOI: 10.1016/j.ijrobp.2007.10.059. [6] Yoshii K, Song JY, Park SB, et al. Tick-borne encephalitis in Japan, Republic of Korea and China [J]. Emerg Microbes Infect, 2017, 6(9): e82. DOI: 10.1038/emi.2017.69. [7] Li XH, Ji HW, Wang D, et al. Molecular detection and phylogenetic analysis of tick-borne encephalitis virus in ticks in northeastern China [J]. J Med Virol, 2022, 94(2): 507-513. DOI: 10.1002/jmv.27303. [8] Wang ZD, Wang B, Wei F, et al. A new segmented virus associated with human febrile illness in China [J]. N Engl J Med, 2019, 380(22): 2116-2125. DOI: 10.1056/NEJMoa1805068. [9] Wang ZD, Wang W, Wang NN, et al. Prevalence of the emerging novel Alongshan virus infection in sheep and cattle in Inner Mongolia, northeastern China [J]. Parasit Vectors, 2019, 12(1): 450. DOI: 10.1186/s13071-019-3707-1. [10] Madison-Antenucci S, Kramer LD, Gebhardt LL, et al. Emerging tick-borne diseases [J]. Clin Microbiol Rev, 2020, 33(2): e00083-e00018. DOI: 10.1128/cmr.00083-18. [11] 刘勇, 蒋彤, 葛婉淇, 等. 东北地区重要蜱虫种类与蜱传疾病研究进展[J]. 中华卫生杀虫药械, 2023, 29(2): 176-179. DOI: 10.19821/j.1671-2781.2023.02.021.Liu Y, Jiang T, Ge WQ, et al. Research progress on species of ticks and tick borne diseases in northeast China [J]. Chin J Hyg Insect Equip, 2023, 29(2): 176-179. DOI: 10.19821/j.1671-2781.2023.02.021. [12] 梁慧杰, 王玉梅, 程成, 等. 黑龙江省中俄边境口岸2019―2020年蜱类及其携带病原体监测[J]. 中国口岸科学技术, 2021, 3(10): 17-22.Liang HJ, Wang YM, Cheng C, et al. Tick and tick-borne pathogens surveillance at the Sino-Russian border port in Heilongjiang Province from 2019 to 2020. [J]. China Port Science and Technology, 2021, 3(10): 17-22. [13] 王泽东. 新型分节段黄病毒的发现及其流行病学研究[D]. 北京: 中国农业科学院, 2019.Wang ZD. Discovery of a new segmented flavivirus and its epidemiological study [D]. Beijing: Chinese Academy of Agricultural Sciences, 2019. [14] 李基旭, 赵鑫, 金光星, 等. 吉林延边地区蜱复合感染新塔拉塞维奇立克次体及SFTSV调查研究[J]. 中华疾病控制杂志, 2019, 23(5): 561-566, 572. DOI: 10.16462/j.cnki.zhjbkz.2019.05.014.Li JX, Zhao X, Jin GX, et al. Investigation of free ticks carrying CRT and compound infection with SFTSV in Yanbian Area of Jilin Province [J]. Chin J Dis Control Prev, 2019, 23(5): 561-566, 572. DOI: 10.16462/j.cnki.zhjbkz.2019.05.014. [15] Martins LA, Bensaoud C, Kotál J, et al. Tick salivary gland transcriptomics and proteomics [J]. Parasite Immunol, 2021, 43(5): e12807. DOI: 10.1111/pim.12807. [16] 门晓钰, 杜海莹, 蔡晓静, 等. 我国蜱和人群中TBEV流行情况的Meta分析[J]. 中国人兽共患病学报, 2021, 37(7): 631-638. DOI: 10.3969/j.issn.1002-2694.2021.00.104.Men XY, Du HY, Cai XJ. Meta analysis of TBEV prevalence in ticks and human populations in China [J]. Chinese Journal of Zoonoses, 2021, 37(7): 631-638. DOI: 10.3969/j.issn.1002-2694.2021.00.104. [17] 王迪. 我国东北地区蜱传脑炎病毒(TBEV)流行病学调查与分析[D]. 长春: 吉林农业大学, 2020.Wang D. Epidemiological investigation and analysis of tick-borne encephalitis virus (TBEV) in Northeast China [D]. Changchun: Jilin Agricultural University, 2020. [18] Forghani M, Kovalev S, Khachay M, et al. Identifying new clusterons: application of TBEV analyzer 3.0 [J]. Microorganisms, 2023, 11(2): 324. DOI: 10.3390/microorganisms11020324. [19] Zhang M, Tian JY, Li HY, et al. The comparative genomic analysis provides insights into the phylogeny and virulence of tick-borne encephalitis virus vaccine strain Senzhang [J]. PLoS One, 2022, 17(8): e0273565. DOI: 10.1371/journal.pone.0273565. [20] 李基旭, SHIN-Hyeong Cho, 朴文, 等. 图们江流域中朝俄边境地区长角血蜱中SFTS病毒监测分析[J]. 中华疾病控制杂志, 2019, 23(2): 201-205, 211. DOI: 10.16462/j.cnki.zhjbkz.2019.02.016.Li JX, Cho S, Piao W, et al. Surveillance and analysis of Haemaphysalis Iongicornis with SFTS virus in Tumen River Basin located in the frontiers of Russia, Korea and Northeast China [J]. Chin J Dis Control Prev, 2019, 23(2): 201-205, 211. DOI: 10.16462/j.cnki.zhjbkz.2019.02.016. -
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