Abstract: Objective To investigate the prevalence rate of dental fluorosis of primary and secondary school students in Gulin county and explore the influencing factors. Methods Stratified cluster sampling method was adopted and the unified questionnaire was conducted among primary and secondary school students in light, medium and serious endemic areas of Gulin county. Results The prevalence rate of primary and secondary school students' dental fluorosis was 56.32% in Gulin county, and students aged 8-12 accounted for 50.00%. The pass rate of health knowledge examination was only 37.12%. Age (OR=1.419, 95% CI:1.255-1.605), living in rural areas (OR=1.416,95% CI:1.120-1.790) or serious endemic areas (OR=1.747,95% CI:1.600-1.906) were major risk factors for the occurrence of dental fluorosis. The better the family economic condition (OR=0.765,95% CI:0.656-0.892) or education background of mothers was(OR=0.899,95% CI:0.828-0.977), the lower prevalence rate of dental fluorosis would be. Conclusions The prevalence rate of dental fluorosis in Gulin is still high, and the influencing factors include age, family economic condition, education background of mother etc. Paying attention to influencing factors and taking comprehensive control measures will make the dental fluorosis prevention and control more effective and sustainable.