Abstract: Objective To investigate the prevalence and risk factors of hepatitis E virus (HEV) infection among the general population in Wuhan. Methods Multi-stage random sampling method was adopted. A total of 3 890 general population aged 0 to 59 years were sampled, their serum samples were also collected and detected for anti-HEV IgG by ELISA. Chi-square test, exact binomial formula and unconditional logistic regression analysis were performed for statistical analysis. Results The standardized seroprevalence of anti-HEV IgG was 35.01%, which cumulated with age. The seroprevalence between male and female had no significant difference(χ²=3.18，P=0.075), the seroprevalence of elder (χ²=542.32，P<0.001)and residents in urban (χ²=11.32，P=0.001) were higher. Multivariate unconditional logistic regression analysis was performed among people over 18 years old, it showed that the male, elder (aged 30 to 59 years), urban residents and people having a preference of fish were at higher risk of HEV infection (all P<0.050). Conclusions The infection rate of HEV was high among the general population in Wuhan. The high-risk population were the male, elder (aged 30 to 59 years), urban residents and people having a preference of fish.