摘要:
目的 建立青春期雌性小鼠抑郁模型，为女性青春期抑郁症高发机制研究奠定基础。方法 C57BL/6J 18天雌性、雄性小鼠各16只分别随机分为雌性对照组和雌性组，雄性对照组和雄性组 （n=8）。4组小鼠适应2 d后于第20天测试行为学。雌性组和雄性组自第21天制备3周慢性轻度不可预知应激 （chronic unpredictable mild stress，CUMS） 模型，3周后再对4组进行行为学测试。结果 造模前4组行为学均无统计学差异 （均有P> 0.05）。造模后雌性组较雌性对照组体质量变化、糖水消耗百分比、旷场运动距离及中央区时间、高架十字迷宫开放臂时间减少 （均有P<0.05），悬尾和强迫游泳不动时间增加（均有P<0.05） ；雄性组较雄性对照组体质量变化、糖水消耗百分比、旷场运动距离及中央区时间减少，强迫游泳不动时间增加 （均有P<0.05）。与造模前比较，雌性组糖水消耗百分比和中央区时间均减少 （均有P<0.05），悬尾和强迫游泳不动时间均增加（均有P<0.05）；雄性组较造模前糖水消耗百分比减少（t=6.02，P<0.001），强迫游泳不动时间增加 （t=2.91，P=0.013）。造模后雌性组较雄性组运动距离和开放臂时间均减少 （均有P<0.05），悬尾和强迫游泳不动时间均增加（均有P<0.05）。结论 成功造模后雌性组较雄性组抑郁样行为更加明显，表明青春期雌性小鼠比雄性小鼠对CUMS更加易感。
Abstract:
Objective To establish the depressive model of adolescent female mice and lay a foundation for the mechanism investigation of rapidly increased incidence of adolescent female depression. Methods 16 C57BL/6J female and 16 male mice (P18) were randomly chosen into female control group, female group, male control group and male group respectively (n=8). Behavioral tests were taken for all mice (P20) after 2 days' adaptive phase. Chronic unpredictable mild stress (CUMS) model for 3 weeks was taken for the mice (P21) of female and male groups and then behavioral tests were taken again for all the mice (P41) of 4 groups after 3 weeks. Results There was no significant difference on the behavioral tests results among 4 groups before CUMS (all P>0.05). After 3-week CUMS, change degree of body weight, saccharin consumption percentage, the travelled distance and time spent in center in open field test and the time in open arms in elevated plus maze were decreased (all P<0.05) and the immobility time of tail suspension test and forced swimming test were increased (all P<0.05) in female group compared with female control group. After 3-week CUMS, change degree of body weight, saccharin consumption percentage, the travelled distance and time spent in center in open field test were decreased and the immobility time of forced swimming test was increased (all P<0.05) in male group compared with male control group. After CUMS, saccharin consumption percentage and the time spent in center were decreased (all P<0.05), while the immobility time of tail suspension test and forced swimming test were increased (all P<0.05) in female group compared with those before CUMS. After CUMS, saccharin consumption percentage was less (t=6.02，P<0.001) and the immobility time of forced swimming test was longer (t=2.91，P=0.013) in male group than those before CUMS. After CUMS, the travelled distance in open field test and the time in open arms in elevated plus maze were less (all P<0.05) and the immobility time of tail suspension test and forced swimming test was longer (all P<0.05) in female group than male group. Conclusions The results showed that the depressive-like behaviors in female group were obviously more severe than those in male group after establishing the depressive model successfully. It is possible that adolescent female mice are more susceptible to CUMS than adolescent male mice.
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