2015年湖北地区诺如病毒流行特点及基因型别特征

李静; 张婷; 徐军强; 占建波; 官旭华; 邢学森; 马静; 邱德志; 黄静; 郭配歌; 宋毅

doi:10.16462/j.cnki.zhjbkz.2017.04.007

2015年湖北地区诺如病毒流行特点及基因型别特征

doi: 10.16462/j.cnki.zhjbkz.2017.04.007

李静¹,
张婷¹,
徐军强¹,
占建波¹,
官旭华¹,
邢学森¹,
马静²,
邱德志³,
黄静⁴,
郭配歌⁵,
宋毅^1, ,

1. 湖北省疾病预防控制中心卫生检验所, 湖北武汉 430079;
2. 宜昌市疾病预防控制中心病原微生物检验所, 湖北宜昌 420500;
3. 襄阳市疾病预防控制中心急传科, 湖北襄阳 420600;
4. 恩施市疾病预防控制中心急传科, 湖北恩施 422801;
5. 汉川市疾病预防控制中心急传科, 湖北汉川 420984

详细信息

作者简介:
李静(1973-),女,湖北武汉人,副主任技师,博士。主要研究方向:分子病毒学和分子流行病学。

中图分类号: R442.2;R181.2
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出版历程
- 收稿日期: 2016-11-13
- 修回日期: 2017-01-15

Epidemic characteristics and genotypes of norovirus in Hubei Province, 2015

1. Institute of Health Examination, Hubei Province Center for Disease Control and Prevention, Wuhan 430079, China;
2. Pathogenic Microorganism Detection Department, Yichang City Center for Disease Control and Prevention, Yichang 420500, China;
3. Acute Infectious Disease Department, Xiangyang City Center for Disease Control and Prevention, Xiangyang 420600, China;
4. Acute Infectious Disease Department, Enshi City Center for Disease Control and Prevention, Enshi 422801, China;
5. Acute Infectious Disease Department, Hanchuan City Center for Disease Control and Prevention, Hanchuan 420984, China

摘要

摘要: 目的了解湖北地区腹泻患者诺如病毒（norovirus，NV）的流行病学特征及基因型别特征。方法收集2015年1~12月儿童和成人腹泻患者的粪便标本687份，采用Real-time逆转录聚合酶链式反应（reverse transcription-polymerase chain reavtions，RT-PCR）方法进行NV的核酸检测；应用RT-PCR方法对NV检测阳性的GⅡ型标本再行扩增ORF 2基因 N/S区基因片段后，将该PCR产物直接测序并进行基因型别和系统发育分析。结果 687份腹泻患者粪便标本中男性感染者93例，女性感染者56例，性别差异无统计学意义（χ²=1.193，P=0.275）。患者主要为5岁及以下儿童。2015年湖北地区NV感染全年均有发生，但流行高峰出现在9月份；NV主要为GⅡ基因型，仅检出2例GI基因型。系统发育分析显示，NV GⅡ流行株可分为5种基因亚型，分别为GⅡ.17、GⅡ.4 Sydney 2012、GⅡ.3、GⅡ.6和GⅡ.13，不同基因亚型的流行呈现月份更替现象。结论 2015年湖北地区存在多种基因型 NV的感染，加强NV的分子流行病学监测有利于NV的科学防控。
- 诺罗病毒 /
- 流行病学 /
- 基因型
Abstract: Objective To describe the epidemiologic characteristics and genotypes of norovirus (NV) infection surveillance in Hubei Province in 2015. Methods 687 stool samples from children and adults with diarrhea were collected from January to December in 2015. NV was detected using real-time reverse transcription-polymerase chain reactions (real-time RT-PCR). Positive samples of NV GⅡ were amplified to obtain the N/S region products by RT-PCR. The PCR products were sequenced for genotype identification and phylogenetic analysis. Results The infected male and female were 93 and 56 respectively and there was no significant difference between men and women (χ²=1.193,P=0.275). The infected population was mainly under 5 years old. NV infections occurred through 2015 and the epidemic peak was in September. The main genotype of NV was GⅡ in Hubei and only 2 strains was GI. The phylogenetic analysis showed that NV GⅡ strains were identified as 5 subgenotypes, GⅡ.17, GⅡ.4 Sydney 2012, GⅡ.3, GⅡ. 6 and GⅡ.13. The different genetic subtype predominated by months in turn. Conclusions There were various subgenotypes of NV among infected population in Hubei Province, 2015. Enhancing the molecular epidemiological surveillance was crucial for NV scientific prevention.
- Norovirus /
- Epidemiology /
- Genotype