Abstract: Objective To explore the association of SLC22A12 gene rs893006 and SLC2A9 gene rs11942223 with hyperuricemia and provide the evidences for the population genetics research and hyperuricemia prevention and treatment. Methods 364 subjects were selected in a certain medical institution as case group, meanwhile, 364 subjects were selected as control group matching for the gender, ethnicity and age. Sequenom Mass ARRAY iPLEX GOLD technology was used to analyze the single nucleotide polymorphisms (SNPs) site genotype of case and control groups, multifactor dimensionality reduction (MDR) was used to analyze the interaction relationship between genetic polymorphisms of rs893006, rs11942223 and body mass index (BMI) in hyperuricemia population. Results The creatinine, total cholesterol, triglyceride, systolic blood pressure and BMI of case group were and allele frequency higher than control, there were significant difference in two groups (all P<0.05). rs893006 SNPs site genotype frequency and allele frequency were significantly different in two groups among male (χ₁²=6.372, P=0.041;χ₂²=4.935,P=0.026). In the different genetic model of male group, G allele of rs893006 SNP site was a protective factor, the hyperuricemia incidence of GT and GG carriers was 0.405 times than TT carrier. The interaction analysis between genes and BMI showed weak synergy effect between rs893006, rs11942223 and BMI, while an obvious antagonistic effect existed between rs893006 and rs11942223, high-risk genotype carriers had higher risk for hyperuricemia than low-risk genotype (OR=1.99, χ²=12.499,P<0.001). Conclusions SLC22A12 gene rs893006 SNP site was associated with the incidence of hyperuricemia in male group. There was an interaction effect between rs893006, rs11942223 and BMI.