摘要:
目的 探究过氧化物酶增殖体激活受体γ（peroxisome proliferator-activatedreceptor γ，PPARγ）激动剂对妊娠期糖尿病（gestational diabetes mellitus，GDM）小鼠体内氧化应激和炎症水平的影响。方法 7周C57bl/6J小鼠建立GDM模型，选取24只随机均分：GDM模型组和罗格列酮（rosiglitazone，RSG）干预组。选12只孕鼠作空白对照组。记录受孕日期（gestation day，GD）及GD10~GD18孕鼠体重及血糖。葡萄糖氧化酶-过氧化物酶法测定血糖；放射免疫法（radioimmunoassay，RIA）测血清胰岛素水平；酶联免疫吸附法测定孕鼠血清肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）、白介素-6（interleukin-6，IL-6）、白介素-8（interleukin-8，IL-8）水平；WST-1法测定超氧化物歧化酶含量（superoxide dismutase，SOD）；过氧化物酶微量测定法检测谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px）的含量。结果 不同时间段的孕鼠体重（F=306.53，P<0.001），血糖（F=31.20，P<0.001）差异有统计学意义；时间与组别交互效应的影响差别均具有统计学意义（均有P<0.05）。各组孕鼠胰岛素水平差异具有统计学意义（F=6.33，P=0.009）；各组孕鼠血清SOD、GSH-Px水平及炎症因子（TNF-α、IL-6、IL-8）水平差异均有统计学意义（均有P<0.05）。结论 PPARγ激动剂可升高GDM小鼠血清SOD、GSH-Px水平，降低TNF-α水平，改善GDM小鼠体内氧化应激及炎症水平。
Abstract:
Objective To explore the effect of peroxisome proliferator-activated receptor γ (PPARγ) agonist on the level of oxidative stress and inflammation in gestational diabetes mellitus (GDM) mice. Methods The maternal gestational diabetes mouse model was established in 7-week-old C57/BL6 mice. Twenty-four GDM model mice were divided randomly into two subgroups:GDM group and rosiglitazone (RSG) intervention group. Twelve pregnant mice were selected as control group. The data of weight and glucose were detected on gestation day (GD) 10,12,14,16 and 18. Glucose oxidase-peroxidase method was used to determine the glucose levels in the blood, determination of serum insulin levels by radioimmunoassay (RIA), serum tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and interleukin-8(IL-8) levels were measured by enzyme linked immunosorbent assay, serum superoxide dismutase (SOD) activity levels were measured by WST-1, determination of glutathione peroxidase (GSH-Px) content by peroxidase micro assay. Results The body weight(F=306.53,P<0.001)and serum glucose (F=31.20,P<0.001) of pregnant mice were significantly different at different time (all P<0.05). The body weight and serum glucose were also affected by interaction of time and group(all P<0.05). The insulin levels of pregnant mice in each group were significantly different (F=6.33,P=0.009). The levels of oxidative stress (SOD, GSH-Px) and inflammatory factors (TNF-α, IL-6 and IL-8) in each group were significantly different (all P<0.05). Conclusions PPARγ agonist supplement can increase the levels of SOD and GSH-Px, and reduce the levels of TNF-α to improve the environment of oxidative stress and inflammation in gestational diabetes mellitus mice.
点击查看大图
下载: