HCVF蛋白和负性共刺激分子2B4与慢性HCV感染的关联研究

石丽萍; 李森森; 肖雯; 裴家平; 季晓伟; 蒋龙凤; 王长军; 邓小昭; 张琪; 韩一芳; 张锦海

doi:10.16462/j.cnki.zhjbkz.2018.06.017

HCVF蛋白和负性共刺激分子2B4与慢性HCV感染的关联研究

doi: 10.16462/j.cnki.zhjbkz.2018.06.017

1. 南京医科大学基础医学院生物化学与分子生物学系, 江苏南京 210029;
2. 中国药科大学生命科学与技术学院微生物与生化药学系, 江苏南京 210009;
3. 南京军区疾病预防控制中心疾病预防控制所, 江苏南京 210002;
4. 南京医科大学第一附属医院感染病科, 江苏南京 210029

基金项目:

国家自然科学基金（81573213）；中国肝炎基金会天晴肝病研究基金（CFHPC20132071）；江苏省自然科学基金（BK20151089，BK20161059，BL2013021）

详细信息

作者简介:
石丽萍(1993-),女,江苏常州人,在读硕士研究生。主要研究方向:病毒性肝炎发病机制。

中图分类号: R373.21
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出版历程
- 收稿日期: 2017-12-27
- 修回日期: 2018-03-27

Association between F protein and negative costimulatory molecule 2B4 in chronic HCV infection

1. Department of Biochemistry and Molecular Biology, School of Basic Medicine, Nanjing Medical University, Nanjing 210029, China;
2. Department of Microbial and Biochemical Pharmacy, School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China;
3. Institute of Disease Control and Prevention, Center for Disease Control and Prevention of Nanjing Command, Nanjing 210002, China;
4. Department of Infection, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China

摘要

摘要: 目的探讨丙型肝炎病毒（hepatitis C virus，HCV）F蛋白的产生与负性共刺激分子2B4在慢性HCV感染中的关联性。方法收集慢性HCV患者（chronic hepatitis patient，CHP）的血液样本，检测F抗体（F antibody，F-Ab）的阳性率并依据结果分成（HCV-F（+）组、HCV-F（-）组）两组，收集健康者的血液样本作为对照组；分离并培养外周血单核细胞（peripheral blood mononuclear cells，PBMCs），收集各孔细胞，酶联免疫吸附试验检测2B4抗体阻断前后白细胞介素4（interleukin 4，IL-4）、干扰素γ（interferon γ，IFN-γ）的表达水平。结果 2B4抗体阻断前，HCV患者PBMCs中IFN-γ和IL-4分泌水平均较健康组高（均有P<0.05），且HCV-F（-）组分泌IFN-γ水平高于HCV-F（+）组（F=1.908，P=0.020），而IL-4分泌水平低于HCV-F（+）组（F=1.342，P=0.009）。2B4抗体阻断后，健康组中IFN-γ和IL-4水平较阻断前均无统计学意义（均有P>0.05），CHP IFN-γ分泌水平较阻断前升高（F=1.214，P=0.003），且HCV-F（-）组升高程度高于HCV-F（+）组（F=1.434，P=0.009）；而IL-4分泌水平较阻断前明显降低（F=1.505，P=0.015），且HCV-F（-）组降低程度低于HCV-F（+）组（F=1.444，P=0.032）。结论在慢性HCV感染中，F蛋白的信号调控机制与负性共刺激分子2B4具有相关性。
- 丙型肝炎病毒F蛋白 /
- 负性共刺激分子2B4 /
- 丙型肝炎病毒感染
Abstract: Objective To investigate the relationship between the production of hepatitis C virus (HCV) F protein and the negative costimulatory molecule 2B4 in chronic HCV infection. Methods The blood samples of chronic hepatitis patients (CHP) were collected and the positive rate of F antibody (F-Ab) was detected and divided into HCV-F(+) and HCV-F(-). Blood samples collected from healthy volunteers were used as control group. Peripheral blood mononuclear cells (PBMCs) were isolated and cultured.The levels of interleukin 4 (IL-4),interferon γ (IFN-γ) before and after the blockade of 2B4 antibody were detected by enzyme-linkead immunosorbent assay. Results The levels of IFN-γ and IL-4 secreted in HCV patients were significantly higher than those in healthy controls (all P<0.05), and the level of IFN-γ secreted in HCV-F (-) group was significantly higher than that of HCV-F (+) group (F=1.908,P=0.020), while the level of IL-4 secretion was lower than that of HCV-F (+) group (F=1.342,P=0.009). After blocking with 2B4 antibody, the level of IFN-γ and IL-4 in healthy group had no significant change (all P>0.05). The level of IFN-γ in CHP group was significantly higher than that in the control group (F=1.214,P=0.003), and the levels in HCV-F (-) was higher than that of HCV-F (+) group (F=1.434,P=0.009). The levels of IL-4 were significantly lower than those before antibody block (F=1.505,P=0.015), and the levels in HCV-F (-) group were lower than those in HCV-F (+) group (F=1.444,P=0.032). Conclusions In chronic HCV infection, the signal regulation mechanism of F protein is correlated with negative costimulatory molecule 2B4.
- HCV F protein /
- Negative costimulatorymolecule 2B4 /
- HCV infection