Investigation on neutralizing antibody level of Japanese encephalitis virus in adults from Guizhou Province
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摘要:
目的 调查贵州省成人流行性乙型脑炎（乙脑）病毒中和抗体水平。 方法 采用多阶段随机抽样的方法，在2017年5~6月采集3个市（州）6个年龄组健康成人血清共360份，应用蚀斑减少中和试验法检测乙脑病毒中和抗体。 结果 360名调查对象中乙脑病毒中和抗体阳性率为55.28%，几何平均滴度（GMT）为1 ∶17.52。不同性别（χ2=10.798，P=0.001）、不同发病率地区（χ2=6.090，P=0.048）成人乙脑病毒中和抗体阳性率差异具有统计学意义（均有P<0.05）；不同年龄成人乙脑病毒中和抗体阳性率在45.00%~60.00%之间（χ2=4.236，P=0.516）；不同民族中，布依族成人乙脑病毒中和抗体阳性率最高（79.17%）。 结论 贵州省成人乙脑病毒中和抗体水平较低，存在成人乙脑流行风险。 Abstract:Objective To investigate the level of neutralizing antibodies against Japanese encephalitis (JE) virus in adults from Guizhou Province. Methods A multi-stage random sampling method was used to collect 360 serum of healthy adults in 6 age groups of 3 cities (states) from May to June 2017. Neutralizing antibodies against Japanese encephalitis virus of healthy adults were detected by plaque reduction neutralization test. Results The positive rate of neutralizing antibodies against Japanese encephalitis virus was 55.28%, and the geometric mean titer (GMT) was 1:17.52 in 360 subjects. The difference of the positive rates of neutralizing antibodies against Japanese encephalitis virus of adults in different genders (χ2=10.798, P=0.001) and in different incidence regions(χ2=6.090, P=0.048)was statistically significant(both P<0.05). The positive rate of different age groups was 45.00%-60.00% (χ2=4.236, P=0.516). The positive rate of Buyi nationality was the highest (79.17%). Conclusions The level of neutralizing antibodies against Japanese encephalitis virus of adults in Guizhou province is low, and there is a risk of epidemic encephalitis in adults. -
表 1 贵州省健康成人乙脑中和抗体水平的性别分布
Table 1. Gender distribution of Japanese encephalitis neutralization antibody level in healthy adults in Guizhou Province
性别 检测数 阳性数 阳性率(%) GMT(1:) 男 180 84 46.67 17.96 女 180 115 63.89 17.20 
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表 2 贵州省健康成人乙脑中和抗体水平的年龄分布
Table 2. Age distribution of Japanese encephalitis neutralization antibody level in healthy adults in Guizhou Province
年龄组(岁) 检测数 阳性数 阳性率(%) 中和抗体滴度 GMT(1:) <1 ∶10 1 ∶10 1 ∶20 1 ∶40 >1 ∶40 16~ 60 36 60.00 24 21 6 5 4 18.52 21~ 60 27 45.00 33 17 8 2 0 13.61 31~ 60 31 51.67 29 18 9 2 2 15.99 41~ 60 35 58.33 25 17 9 6 3 18.85 51~ 60 36 60.00 24 15 12 8 1 18.52 >60 60 34 56.67 26 18 4 9 3 19.20 合计 360 199 55.28 161 106 48 32 13 17.52
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表 3 贵州省不同发病率地区健康成人乙脑中和抗体水平的分布
Table 3. Different incidence region distribution of Japanese encephalitis neutralization antibody level in healthy adults in Guizhou Province
发病率 检测数 阳性数 阳性率(%) 中和抗体滴度 GMT(1:) <1 ∶10 1 ∶10 1 ∶20 1 ∶40 >1 ∶40 高 120 57 47.50 63 35 13 6 3 15.49 中 120 76 63.33 44 41 14 13 8 18.94 低 120 66 55.00 54 30 21 13 2 17.82
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表 4 贵州省健康成人乙脑中和抗体水平的民族分布
Table 4. Nation distribution of Japanese encephalitis neutralization antibody level in healthy adults in Guizhou Province
民族 检测数 阳性数 阳性率(%) GMT(1:) 汉 167 94 56.29 19.42 苗 68 35 51.47 15.46 水 52 24 46.15 17.31 布依 24 19 79.17 14.40 其他 49 27 55.10 16.71
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