Toll样受体2基因多态性与青海地区结核病易感性的相关性研究

赵昌明; 王玉清; 黄秋丽; 王兴斌; 闫晓萍; 王玲; 刘旷怡; 李斌; 苏效东; 张亚欣; 杨得杰; 王兆芬

doi:10.16462/j.cnki.zhjbkz.2024.02.014

Toll样受体2基因多态性与青海地区结核病易感性的相关性研究

doi: 10.16462/j.cnki.zhjbkz.2024.02.014

1.
青海大学医学部公共卫生系，西宁 810001
2.
青海省第四人民医院呼吸一科，西宁 810007

基金项目:

青海省自然科学基金 2020-ZJ-929

详细信息

通讯作者:
王兆芬，E-mail: kristy538@163.com

中图分类号: R181.3
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出版历程
- 收稿日期: 2023-03-22
- 修回日期: 2023-07-04
- 网络出版日期: 2024-03-30
- 刊出日期: 2024-02-10

Correlation between TLR2 gene polymorphism and tuberculosis susceptibility in Qinghai Province

1.
Department of Public Health, Medical School, Qinghai University, Xining 810001, China
2.
Respiratory Department I, the 4th People′s Hospital of Qinghai Province, Xining 810007, China

Funds:

Natural Science Foundation of Qinghai Province 2020-ZJ-929

More Information

Corresponding author: WANG Zhaofen, E-mail: kristy538@163.com

摘要

摘要: 目的研究Toll样受体2(Toll-like receptors-2, TLR 2)基因rs3804099、rs3804100位点的多态性与青海地区结核病的关系。方法收取青海省某医院219例结核病患者为病例组，236例同期体检者为对照组，采用聚合酶链式反应(polymerase chain reaction, PCR)技术扩增rs3804099、rs3804100位点并鉴定基因型。结果 TLR2基因rs3804099(χ² =4.37, P=0.037)和rs3804100(χ² =4.73, P=0.030)位点基因多态性与结核病相关；在基因模型rs3804099(χ² =5.23, P=0.022)、rs380410(χ² =7.70, P=0.006)位点在杂合子模型中两组间比较差异均有统计学意义；rs3804100位点在隐形模型中两组间有统计学差异(χ² =4.92, P=0.027)。TLR2基因rs3804099(χ² =4.24, P=0.040)和rs3804100(χ² =5.19, P=0.023)位点基因多态性与藏族人群结核病易感性相关，但未发现该位点与汉族人群结核病的发病相关。结论 TLR2基因rs3804099、rs3804100位点基因多态性与青海地区藏族人群结核病的发病相关，CC基因型可能是结核病易感性的一个危险性因素。
- 结核病 /
- Toll样受体基因 /
- 基因多态性 /
- 易感性 /
- 肺结核
Abstract: Objective To study the relationship between rs3804099 and rs3804100 polymorphisms of Toll-like receptors-2(TLR2) gene and tuberculosis in Qinghai Province. Methods 219 tuberculosis patients from a hospital in Qinghai Province were collected as the case group, and 236 physical examinees at the same time as the control group. The rs3804099 and rs3804100 loci were amplified by polymerase chain reaction (PCR) and genotypes were determined by sequencing. Results rs3804099 (χ² =4.37, P=0.037) and rs3804100 (χ² =4.73, P=0.030) polymorphisms in TLR2 were associated with tuberculosis. The model analysis showed, rs3804099(χ² =5.23, P=0.022) and rs3804100(χ² =7.70, P=0.006) loci were statistically different in the heterozygote model between two groups; while only rs3804100 loci was significantly different in the invisible model (χ² =4.92, P=0.027). The rs3804099(χ² =4.24, P=0.040) and rs3804100(χ² =5.19, P=0.023) polymorphisms of TLR2 gene were correlated with tuberculosis susceptibility in Tibetan population; ), but no correlation was found in Han population. Conclusions The rs3804099 and rs3804100 polymorphisms of TLR2 gene are associated with occurence of tuberculosis in Tibetan in Qinghai, and CC genotype may be a risk factor for causing tuberculosis.
- Tuberculosis /
- Toll-like receptors gene /
- Gene polymorphism /
- Susceptibility /
- Pulmonary tuberculosis

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图 1 Toll样受体2各位点聚合酶链式反应产物电泳结果

A: rs3804099位点；B: rs3804100位点。

Figure 1. Electrophoresis results of Toll-like receptors-2 polymerase chain reaction products at each site

A: rs3804099 site; B: rs3804100 site.

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图 2 Toll样受体2基因测序图

A: rs3804099位点；B: rs3804100位点；箭头表示突变位点。

Figure 2. Sequencing map of Toll-like receptors-2 gene

A: rs3804099 site; B: rs3804100 site; Arrows indicate mutation sites.

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表 1 Toll样受体2基因各位点引物序列

Table 1. Toll-like receptors-2 gene point primer sequence

引物名称 Primer names	引物序列(5′-3′) Primer Sequence(5′-3′)	片段长度 Product Size (bp)
rs3804099-F	TGCAAATCCTGAGAGTGGGA	237
rs3804099-R	GCAGTTCCAAACATTCCACGG
rs3804100-F	CCGGAGAGACTTTGCTCACT	285
rs3804100-R	TGGGTAAGAGGGAGGCATCT

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表 2 Toll样受体2基因各位点在病例组和对照组分布情况

Table 2. Repartition of Toll-like receptors-2 gene gene loci in the case group and control group

单核苷酸多态性 Single nucleotide polymorphisms	类别 Categories		病例组人数(占比/%) Number of cases (proportion/%) (n=219)	对照组人数(占比/%) Number of controls (proportion/%) (n=236)	χ²值 value	OR值value (95% CI)	P值 value
rs3804099	基因型 Genotype	TT	131(59.82)	125(52.97)		1.000
		TC	69(31.51)	100(42.37)	4.37	1.519(1.026~2.249)	0.037
		CC	19(8.68)	11(4.66)	1.59	0.607(0.278~1.326)	0.207
	等位基因 Alleles	T	331(75.57)	350(74.15)	0.24	1.078(0.799~1.455)	0.622
		C	107(24.43)	122(25.85)
	基因模型 Genetic model	TT vs CC	131/19	125/11	1.59	0.607(0.278~1.326)	0.207
		TC∶CC	69/19	100/11	5.23	0.399(0.179~0.892)	0.022
		TT∶TC+CC	131/88	125/111	2.17	1.322(0.911~1.917)	0.141
		TT+TC∶CC	200/19	225/11	2.97	0.515(0.239~1.108)	0.085
rs3804100	基因型 Genotype	TT	136(62.10)	131(55.51)		1.000
		TC	65(29.68)	97(41.10)	4.73	1.549(1.043~2.300)	0.030
		CC	18(8.22)	8(3.39)	3.18	0.461(0.194~1.098)	0.075
	等位基因 Alleles	T	339(77.4)	359(76.06)	0.23	1.078(0.792~1.467)	0.633
		C	99(22.6)	113(23.94)
	基因模型 Genetic model	TT∶CC	136/18	131/8	3.18	0.461(0.194~1.098)	0.075
		TC∶CC	65/18	97/8	7.07	0.298(0.122~0.725)	0.006
		TT∶TC+CC	136/83	131/105	2.04	1.313(0.903~1.910)	0.154
		TT+TC∶CC	201/18	228/8	4.92	0.392(0.167~0.921)	0.027

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表 3 不同民族等位基因和基因型分布情况

Table 3. Distribution of alleles and genotypes in different ethnic groups

单核苷酸多态性 Single nucleotide polymorphisms	民族 Ethnic groups	基因型 Genotype	病例组人数(占比/%) Number of cases (proportion/%) (n=219)	对照组人数(占比/%) Number of controls (proportion/%) (n=236)	χ²值 value	OR值value (95% CI)	P值 value
rs3804099	汉族 Han	TT	40(18.26)	35(14.83)		1.000
		TC	29(13.24)	34(14.41)	0.730	1.340(0.684~2.623)	0.393
		CC	5(2.28)	3(1.27)	0.712	0.686(0.153~3.078)	0.456
		T	109(24.89)	104(22.03)	0.08	1.075(0.641~1.802)	0.784
		C	39(8.90)	40(8.47)
	藏族 Tibetans	TT	91(41.55)	90(38.14)		1.000
		TC	40(18.26)	66(27.97)	4.237	1.668(1.023~2.721)	0.040
		CC	14(6.39)	8(3.39)	1.402	0.578(0.231~1.444)	0.236
		T	222(50.68)	246(52.12)	0.20	1.088(0.752~1.574)	0.653
		C	68(15.53)	82(17.37)
rs3804100	汉族 Han	TT	43(19.63)	36(15.25)		1.000
		TC	27(12.33)	33(13.98)	1 213	1.460(0.744~2.865)	0.271
		CC	4(1.83)	3(1.27)	1.00	0.896(0.188~4.268)	0.605
		T	113(25.80)	105(22.25)	0.46	1.199(0.707~2.033)	0.500
		C	35(7.99)	39(8.26)
	藏族 Tibetans	TT	95(43.38)	95(40.25)		1.000
		TC	36(16.44)	64(27.12)	5.19	1.778(1.081~2.924)	0.023
		CC	14(6.39)	5(2.12)	3.88	0.357(0.124~1.031)	0.049
		T	226(51.60)	254(53.81)	0.02	1.029(0.704~1.504)	0.883
		C	64(14.61)	74(15.68)

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