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Toll样受体2基因多态性与青海地区结核病易感性的相关性研究

赵昌明 王玉清 黄秋丽 王兴斌 闫晓萍 王玲 刘旷怡 李斌 苏效东 张亚欣 杨得杰 王兆芬

赵昌明, 王玉清, 黄秋丽, 王兴斌, 闫晓萍, 王玲, 刘旷怡, 李斌, 苏效东, 张亚欣, 杨得杰, 王兆芬. Toll样受体2基因多态性与青海地区结核病易感性的相关性研究[J]. 中华疾病控制杂志, 2024, 28(2): 215-220. doi: 10.16462/j.cnki.zhjbkz.2024.02.014
引用本文: 赵昌明, 王玉清, 黄秋丽, 王兴斌, 闫晓萍, 王玲, 刘旷怡, 李斌, 苏效东, 张亚欣, 杨得杰, 王兆芬. Toll样受体2基因多态性与青海地区结核病易感性的相关性研究[J]. 中华疾病控制杂志, 2024, 28(2): 215-220. doi: 10.16462/j.cnki.zhjbkz.2024.02.014
ZHANG Changming, WANG Yuqing, HUANG Qiuli, WANG Xingbin, YAN Xiaoping, WANG Ling, LIU Kuangyi, LI Bin, SU Xiaodong, ZHANG Yaxin, YANG Dejie, WANG Zhaofen. Correlation between TLR2 gene polymorphism and tuberculosis susceptibility in Qinghai Province[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2024, 28(2): 215-220. doi: 10.16462/j.cnki.zhjbkz.2024.02.014
Citation: ZHANG Changming, WANG Yuqing, HUANG Qiuli, WANG Xingbin, YAN Xiaoping, WANG Ling, LIU Kuangyi, LI Bin, SU Xiaodong, ZHANG Yaxin, YANG Dejie, WANG Zhaofen. Correlation between TLR2 gene polymorphism and tuberculosis susceptibility in Qinghai Province[J]. CHINESE JOURNAL OF DISEASE CONTROL & PREVENTION, 2024, 28(2): 215-220. doi: 10.16462/j.cnki.zhjbkz.2024.02.014

Toll样受体2基因多态性与青海地区结核病易感性的相关性研究

doi: 10.16462/j.cnki.zhjbkz.2024.02.014
基金项目: 

青海省自然科学基金 2020-ZJ-929

详细信息
    通讯作者:

    王兆芬,E-mail: kristy538@163.com

  • 中图分类号: R181.3

Correlation between TLR2 gene polymorphism and tuberculosis susceptibility in Qinghai Province

Funds: 

Natural Science Foundation of Qinghai Province 2020-ZJ-929

More Information
  • 摘要:   目的  研究Toll样受体2(Toll-like receptors-2, TLR 2)基因rs3804099、rs3804100位点的多态性与青海地区结核病的关系。  方法  收取青海省某医院219例结核病患者为病例组,236例同期体检者为对照组,采用聚合酶链式反应(polymerase chain reaction, PCR)技术扩增rs3804099、rs3804100位点并鉴定基因型。  结果  TLR2基因rs3804099(χ2 =4.37, P=0.037)和rs3804100(χ2 =4.73, P=0.030)位点基因多态性与结核病相关;在基因模型rs3804099(χ2 =5.23, P=0.022)、rs380410(χ2 =7.70, P=0.006)位点在杂合子模型中两组间比较差异均有统计学意义;rs3804100位点在隐形模型中两组间有统计学差异(χ2 =4.92, P=0.027)。TLR2基因rs3804099(χ2 =4.24, P=0.040)和rs3804100(χ2 =5.19, P=0.023)位点基因多态性与藏族人群结核病易感性相关,但未发现该位点与汉族人群结核病的发病相关。  结论  TLR2基因rs3804099、rs3804100位点基因多态性与青海地区藏族人群结核病的发病相关,CC基因型可能是结核病易感性的一个危险性因素。
  • 图  1  Toll样受体2各位点聚合酶链式反应产物电泳结果

    A: rs3804099位点;B: rs3804100位点。

    Figure  1.  Electrophoresis results of Toll-like receptors-2 polymerase chain reaction products at each site

    A: rs3804099 site; B: rs3804100 site.

    图  2  Toll样受体2基因测序图

    A: rs3804099位点;B: rs3804100位点;箭头表示突变位点。

    Figure  2.  Sequencing map of Toll-like receptors-2 gene

    A: rs3804099 site; B: rs3804100 site; Arrows indicate mutation sites.

    表  1  Toll样受体2基因各位点引物序列

    Table  1.   Toll-like receptors-2 gene point primer sequence

    引物名称
    Primer names
    引物序列(5′-3′)
    Primer Sequence(5′-3′)
    片段长度
    Product Size (bp)
    rs3804099-F TGCAAATCCTGAGAGTGGGA 237
    rs3804099-R GCAGTTCCAAACATTCCACGG
    rs3804100-F CCGGAGAGACTTTGCTCACT 285
    rs3804100-R TGGGTAAGAGGGAGGCATCT
    下载: 导出CSV

    表  2  Toll样受体2基因各位点在病例组和对照组分布情况

    Table  2.   Repartition of Toll-like receptors-2 gene gene loci in the case group and control group

    单核苷酸多态性
    Single nucleotide polymorphisms
    类别  Categories 病例组人数(占比/%)
    Number of cases (proportion/%) (n=219)
    对照组人数(占比/%)
    Number of controls (proportion/%) (n=236)
    χ2
    value
    OR值value
    (95% CI)
    P
    value
    rs3804099 基因型  Genotype TT 131(59.82) 125(52.97) 1.000
    TC 69(31.51) 100(42.37) 4.37 1.519(1.026~2.249) 0.037
    CC 19(8.68) 11(4.66) 1.59 0.607(0.278~1.326) 0.207
    等位基因  Alleles T 331(75.57) 350(74.15) 0.24 1.078(0.799~1.455) 0.622
    C 107(24.43) 122(25.85)
    基因模型  Genetic model TT vs CC 131/19 125/11 1.59 0.607(0.278~1.326) 0.207
    TC∶CC 69/19 100/11 5.23 0.399(0.179~0.892) 0.022
    TT∶TC+CC 131/88 125/111 2.17 1.322(0.911~1.917) 0.141
    TT+TC∶CC 200/19 225/11 2.97 0.515(0.239~1.108) 0.085
    rs3804100 基因型  Genotype TT 136(62.10) 131(55.51) 1.000
    TC 65(29.68) 97(41.10) 4.73 1.549(1.043~2.300) 0.030
    CC 18(8.22) 8(3.39) 3.18 0.461(0.194~1.098) 0.075
    等位基因  Alleles T 339(77.4) 359(76.06) 0.23 1.078(0.792~1.467) 0.633
    C 99(22.6) 113(23.94)
    基因模型  Genetic model TT∶CC 136/18 131/8 3.18 0.461(0.194~1.098) 0.075
    TC∶CC 65/18 97/8 7.07 0.298(0.122~0.725) 0.006
    TT∶TC+CC 136/83 131/105 2.04 1.313(0.903~1.910) 0.154
    TT+TC∶CC 201/18 228/8 4.92 0.392(0.167~0.921) 0.027
    下载: 导出CSV

    表  3  不同民族等位基因和基因型分布情况

    Table  3.   Distribution of alleles and genotypes in different ethnic groups

    单核苷酸多态性
    Single nucleotide polymorphisms
    民族
    Ethnic groups
    基因型
    Genotype
    病例组人数(占比/%)
    Number of cases (proportion/%) (n=219)
    对照组人数(占比/%)
    Number of controls (proportion/%) (n=236)
    χ2
    value
    OR值value
    (95% CI)
    P
    value
    rs3804099 汉族  Han TT 40(18.26) 35(14.83) 1.000
    TC 29(13.24) 34(14.41) 0.730 1.340(0.684~2.623) 0.393
    CC 5(2.28) 3(1.27) 0.712 0.686(0.153~3.078) 0.456
    T 109(24.89) 104(22.03) 0.08 1.075(0.641~1.802) 0.784
    C 39(8.90) 40(8.47)
    藏族 Tibetans TT 91(41.55) 90(38.14) 1.000
    TC 40(18.26) 66(27.97) 4.237 1.668(1.023~2.721) 0.040
    CC 14(6.39) 8(3.39) 1.402 0.578(0.231~1.444) 0.236
    T 222(50.68) 246(52.12) 0.20 1.088(0.752~1.574) 0.653
    C 68(15.53) 82(17.37)
    rs3804100 汉族  Han TT 43(19.63) 36(15.25) 1.000
    TC 27(12.33) 33(13.98) 1 213 1.460(0.744~2.865) 0.271
    CC 4(1.83) 3(1.27) 1.00 0.896(0.188~4.268) 0.605
    T 113(25.80) 105(22.25) 0.46 1.199(0.707~2.033) 0.500
    C 35(7.99) 39(8.26)
    藏族 Tibetans TT 95(43.38) 95(40.25) 1.000
    TC 36(16.44) 64(27.12) 5.19 1.778(1.081~2.924) 0.023
    CC 14(6.39) 5(2.12) 3.88 0.357(0.124~1.031) 0.049
    T 226(51.60) 254(53.81) 0.02 1.029(0.704~1.504) 0.883
    C 64(14.61) 74(15.68)
    下载: 导出CSV
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出版历程
  • 收稿日期:  2023-03-22
  • 修回日期:  2023-07-04
  • 网络出版日期:  2024-03-30
  • 刊出日期:  2024-02-10

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